Cardiac Troponin I Rapid Test Kit (Lateral Chromatography)
Lintlha tsa sehlahisoa
Tšebeliso e reriloeng:
Cardiac Troponin I Rapid Test Kit e sebelisa colloidal gold immunochromatography ho lemoha pelo ea Troponin I (cTnI) ho serum, plasma kapa sampole eohle ea mali ka boleng kapa semi-quantitatively ka karete e tloaelehileng ea colorimetric.Teko ena e sebelisoa e le thuso ho hlahlobisiseng kotsi ea myocardial joalo ka Acute Myocardial Infarction, Unstable Angina, Acute Myocarditis le Acute Coronary Syndrome.
Melao-motheo ea Teko:
Cardiac Troponin I Rapid Test Kit (Lateral Chromatography) ke mokhoa oa boleng kapa oa halofo, oa membrane e thehiloeng ho immunoassay bakeng sa ho lemoha lefu la pelo la Troponin I(cTnI) maling a feletseng, serum kapa plasma.Ts'ebetsong ena ea tlhahlobo, reagent ea ho ts'oara ha e sebetse sebakeng sa tlhahlobo sa tlhahlobo.Ka mor'a hore mohlala o kenngoe sebakeng sa mohlala oa k'hasete, o itšoara ka likaroloana tse koahetsoeng ke anti-cTnI tlhahlobong.Motsoako ona o falla ka chromatographic ka bolelele ba tlhahlobo mme o sebelisana le "immobilized capture reagent".Sebopeho sa teko se ka lemoha Troponin I(cTnI) ea pelo ka mehlala.Haeba mohlala o na le cardiac Troponin I (cTnI), mola o mebala o tla hlaha sebakeng sa mohala oa teko 'me matla a' mala oa mohala oa teko a eketseha ho ea ka motsoako oa cTnI, o bontšang phello e ntle.Haeba mohlala o se na cardiac Troponin I(cTnI), mohala o mebala o ke ke oa hlaha sebakeng sena, o bontšang sephetho se fosahetseng.E le ho sebetsa e le taolo ea mekhoa, mohala o mebala o tla lula o hlaha sebakeng sa taolo, e leng se bontšang hore molumo o nepahetseng oa mohlala o ekelitsoe 'me ho na le membrane e entsoeng ka membrane.
Litaba Tse ka Sehloohong
Likarolo tse fanoeng li thathamisitsoe tafoleng.
| Karolo REF REF | B032C-01 | B032C-25 |
| K'hasete ea teko | 1 teko | 25 liteko |
| Sehlahisoa sa mohlala | 1 botlolo | 1 botlolo |
| Drophara | 1 sengoathoana | 25 likhomphutha |
| Karete e tloaelehileng ea colorimetric | 1 sengoathoana | 1 sengoathoana |
| Setifikeiti sa ho lumellana | 1 sengoathoana | 1 sengoathoana |
Phallo ea Ts'ebetso
Mohato oa 1: Tokiso ea Mohlala
1. Sesebelisoa sa tlhahlobo se ka etsoa ho sebelisoa mali a feletseng, serum kapa plasma.Sisinya ho khetha serum kapa plasma e le sampole ea teko.Haeba u khetha mali a felletseng e le sampole ea tlhahlobo, a lokela ho sebelisoa hammoho le mokelikeli oa mali.
2. Lekola sampole kareteng ea tlhahlobo hang-hang.Haeba tlhahlobo e ke ke ea phethoa hang-hang, serum le plasma sampole li lokela ho bolokoa ho fihlela matsatsi a 7 ho 2 ~ 8 ℃ kapa li bolokoe ho -20 ℃ bakeng sa likhoeli tse 6 (sampole eohle ea mali e lokela ho bolokoa ho fihlela matsatsi a 3 ho 2 ~ 8 ℃ ) ho fihlela e ka lekoa.
3. Mehlala e tlameha ho khutlisetsoa mocheso oa kamore pele ho hlahlojoa.Ho hlokahala hore lisampole tse hatselitsoeng li qhibilihe ka ho feletseng ebe li tsoakoa hantle pele li etsoa tlhahlobo, ho qoba ho qhoqhoa khafetsa le ho qhibiliha.
4. Qoba ho futhumatsa lisampole, tse ka bakang hemolysis le protheine denaturation.Ho khothalletsoa ho qoba ho sebelisa sampole e matla haholo ea hemolyzed.Haeba sampole e bonahala e na le hemolyzed e matla, sampole e 'ngoe e lokela ho fumanoa le ho hlahlojoa.
Mohato oa 2: Teko
1. Ka kopo bala bukana ea tataiso ka hloko pele u etsa tlhahlobo, khutlisetsa sampole, karete ea tlhahlobo le sampole ea mali e hlapollotsoeng mochesong oa kamore ebe u noa karete.Etsa tlhahiso ea ho bula mokotla oa foil ka mor'a hore o hlaphoheloe mocheso oa kamore le ho sebelisa karete ea teko hang-hang.
2. Beha karete ea teko tafoleng e hloekileng, e behiloeng ka holimo.
Bakeng sa mohlala oa Serum kapa Plasma:
Tšoara dropper ka holimo 'me u fetise marotholi a 3 a serum kapa plasma (hoo e ka bang 80 L, Pipette e ka sebelisoa maemong a tšohanyetso) ho ea ho mohlala hantle , 'me u qale nako.Sheba setšoantšo se ka tlase.
Bakeng sa mohlala oa Whole Blood:
Tšoara dropper ka holimo 'me u fetise marotholi a 3 a mali a feletseng (hoo e ka bang 80 L) ho ea ho mohlala hantle, ebe u eketsa lerotholi le le leng la Sample diluent (hoo e ka bang 40 L), 'me u qale nako.Sheba setšoantšo se ka tlase.
Mohato oa 3: Ho bala
Ka metsotso e 10-30, fumana sephetho sa semi-quantitative ho latela karete e tloaelehileng ea colorimetric ka mahlo.
Ho hlalosa liphetho
E nepahetse: Letoto le le leng le khubelu le hlaha moleng oa taolo (C).Ha e le liphetho tse nepahetseng, o ka fumana semi-quantitative ka mahlo ka karete e tloaelehileng ea colorimetric:
Colour Intensity vs Reference Concentration
| Matla a Mmala | Reference Concentration (ng / ml) |
| - | ＜0.5 |
| + - | 0.5~1 |
| + | 1 ~ 5 |
| + + | 5-15 |
| + + + | 15-30 |
| ++++ | 30 ~ 50 |
| ++++ | ＞50 |
Ha e sebetse: Ha ho purplish red streak e hlahang moleng oa taolo(C).Sena se bolela hore litšoantšiso tse ling li tlameha ho fosahetse kapa karete ea tlhahlobo e se e fosahetse.Boemong bona, ka kopo bala bukana ka hloko hape, 'me u leke hape ka k'hasete e ncha ea teko.Haeba boemo bo tšoanang bo ile ba etsahala hape, u lokela ho khaotsa ho sebelisa sehlopha sena sa lihlahisoa hang-hang 'me u ikopanye le mofani oa thepa ea hau.
Lintlha tsa Odara
| Lebitso la Sehlahiswa | Katse.Che | Boholo | Mohlala | Shelf Life | Trans.& Sto.Temp. |
| Cardiac Troponin I Rapid Test Kit (Lateral Chromatography) | B032C-01 | 1 teko/kit | S/P/WB | Likhoeli tse 24 | 2-30 ℃ |
| B032C-25 | 25 liteko / kit |