Tšebeletso ea Monoclonal Antibody
Monoclonal antibody, e hlahisoang ke B cell clone e le 'ngoe, e na le homogeneity e phahameng haholo e lebisitseng epitope e itseng ea antigen.Monoclonal antibody e na le melemo e mengata, ho kenyelletsa bohloeki bo phahameng, kutlo, le ho hlaka.Li-Hybridomas li entsoe ka ho kopana ha li-lymphocyte tsa B tse hlahisang li-antibodies tse itseng, tse nang le lisele tsa myeloma tsa nako e telele.Bioantibody e sebelisa theknoloji e sebetsang hantle haholo ea ho kopanya e sebetsang ka makhetlo a 20 ho feta mekhoa e tloaelehileng ea ho kopanya.Ntle le moo, e sebelisa theknoloji ea tlhahlobo ea protheine ea microarray ho tsebahatsa li-antibodies tsa monoclonal tse bonts'ang boleng bo phahameng, kamano, le ts'ebetso e sebetsang khahlano le li-epitopes tse itseng.
| Lintho tsa Tšebeletso | Litaba tsa Teko | Nako ea Ketapele (Beke) |
| Tokisetso ea li-antigen | 1. Moreki o fana ka antigen2. Bioantibody e lokisa antigen | / |
| Moento oa litoeba | Ente ea BALB/c mouse, pokello ea serum le tlhahlobo ea ELISA | 4 |
| Ho kopanya lisele le ho hlahloba | Fusion ea li-splenocyte tsa mouse le lisele tsa myeloma, tlhahlobo ea HAT | 2 |
| Ho theha mohala o tsitsitseng oa lisele | Subcloning ea li-clones tse hlahlobiloeng tse ntle | 3 |
| Boitsebiso ba isotype ea antibody | Khethollo ea li-subtypes tsa line line | 1 |
| Ho qhoqhoa ka tekanyo e nyane | Ho kenya serum ntle le serum | 2 |
| E kholo ka tekanyo e incubation le tlhoekiso | 200mL e sa keneng serum le tlhoekiso | 1 |
