Polelo ea Protheine ea Sekokoanyana
Sistimi ea polelo ea lisele tsa likokoanyana ke mokhoa o sebelisoang hangata oa polelo ea eukaryotic bakeng sa ho hlahisa liprotheine tse kholo tsa limolek'hule.Ha ho bapisoa le lisele tsa mammalian, maemo a setso sa lisele tsa likokoanyana a batla a le bonolo 'me ha a hloke CO2.Baculovirus ke mofuta oa kokoana-hloko ea DNA e nang le mefuta e 'meli e nang le lisele tsa likokoanyana e le moemeli oa tlhaho.E na le mefuta e ikhethang ea mefuta, ha e tšoaetse liphoofolo tse nang le lesapo la mokokotlo, 'me ha e kotsi ho batho le liphoofolong.sf9, e sebelisoang haholo joalo ka sele e amohelang batho, e hlaha ka planktonic kapa e khomarela moetlo.sf9 e loketse haholo bakeng sa polelo e kholo, 'me e ka sebelisoa bakeng sa ts'ebetso e latelang le phetoho ea liprotheine tse joalo ka phosphorylation, glycosylation, le acylation.Sistimi ea polelo ea lisele tsa likokoanyana e ka boela ea sebelisoa bakeng sa polelo ea liphatsa tsa lefutso tse ngata, hape e ka hlahisa liprotheine tse chefo joalo ka li-peptide tsa antimicrobial.
| Lintho tsa Tšebeletso | Nako ea ho etella pele (BD) |
| Ntlafatso ea codon, synthesis ea liphatsa tsa lefutso le subcloning | 5-10 |
| P1 molokong o mong ka kokoana-hloko incubation le ponts'o e nyane | 10-15 |
| Ho kenngoa ha kokoana-hloko ea P2, polelo e kholo le tlhoekiso, ho fana ka protheine e hloekisitsoeng le tlaleho ea liteko |