Yeast Cell Protein Expression
Sistimi ea polelo ea tomoso ke mokhoa o sebelisoang haholo bakeng sa polelo ea protheine ea eukaryotic, ka lebaka la ho lema ha eona, ho khoneha le ho sebetsa habonolo.Har'a mefuta e fapaneng ea tomoso, Pichia pastoris ke moamoheli ea tsebahalang haholo oa polelo, kaha e thusa ho hlahisa liprotheine tsa intracellular le extracellular.Sistimi ena e boetse e nolofalletsa liphetoho tsa morao-rao, joalo ka phosphorylation le glycosylation, e hlahisang mokhoa o ikhethang oa polelo ea eukaryotic e nang le melemo e mengata.
| Lintho tsa Tšebeletso | Nako ea ho etella pele (BD) |
| Ntlafatso ea codon, synthesis ea liphatsa tsa lefutso le subcloning | 5-10 |
| Tlhahlobo e ntle ea clone | 10-15 |
| Polelo e nyane | |
| Polelo le tlhoekiso e kholo (200ML), tse ka fanoang li kenyelletsa protheine e hloekisitsoeng le tlaleho ea liteko |
Haeba gene e entsoe ho Bioantibody, plasmid e hahiloeng e tla kenyeletsoa linthong tse ka tsamaisoang.