Prokaryotic Protein Expression
The prokaryotic E. coli expression system e ananeloa ka bophara e le mokhoa o bolokang chelete e ngata, o holileng ka botekgeniki, le o sebelisoang hangata bakeng sa polelo ea protheine.Ho Bioantibody, re ikemiselitse ho fa bareki ba rona lits'ebeletso tse felletseng, tse felletseng ho tloha ho liphatsa tsa lefutso ho isa ho polelo ea protheine le tlhoekiso.Litšebeletso tsa rona li kenyelletsa ntlafatso ea mahala ea codon le ts'ebeliso ea thekenoloji ea rona ea thepa ho rarolla mathata afe kapa afe a amanang le polelo e tlase le ho se qhibilihe ho ka hlahang nakong ea ts'ebetso eohle ea ho itlhalosa le tlhoekiso.Bareki ba rona ba hloka feela ho fana ka mofuta kapa tatellano ea amino acid ea protheine mme re ka fana ka protheine ea boleng bo holimo ka FAST joalo ka libeke tse tharo.Ho feta moo, Bioantibody e fana ka lits'ebeletso tsa ho tlosa endotoxin le ho kenya li-tag tse etselitsoeng litlhoko tse khethehileng tsa bareki ba rona.Re ikemiselitse ho fana ka liphetho mme re ts'episa hore re ke ke ra lefisa litefiso haeba protheine ea ho qetela e sa hlahisoa.
| Lintho tsa Tšebeletso | Litaba tsa Teko | Nako ea ho etella pele (BD) |
| Gene Synthesis | Ntlafatso ea codon, synthesis ea liphatsa tsa lefutso le subcloning. | 5-10 |
| Tlhaloso ea Polelo le Tlhahlobo ea Solubility | 1. Phetoho le ho incubation, ho lemoha maikutlo ka SDS-PAGE.2. Tlhahlobo ea solubility, SDS-PAGE le ho lemoha WB | 10 |
| Ho futhumala ho hoholo le tlhoekiso, protheine ea ho qetela (bohloeki> 85%, 90%, 95%) le tlaleho e tloaelehileng ea liteko | Tlhoekiso ea kamano (Ni kholomo, MBP, GST) |
Haeba gene e entsoe ka hareBioantibody, plasmid e hahiloeng e tla kenyelletsoa ho tse ka tsamaisoang.