Tšebeliso e reriloeng
Sehlahisoa sena se loketse tlhahlobo ea boemo ba kliniki ea serum/plasma/sampole eohle ea mali bakeng sa ho fumanoa ha li-antibodies khahlanong le Leishmania.Ke teko e bonolo, e potlakileng le e se nang lisebelisoa bakeng sa tlhahlobo ea kala-azar e bakoang ke Leishmania.
Teko Molao-motheo
Sehlahisoa sena ke lateral flow chromatographic immunoassay.Khasete ea teko e na le: 1) letlapa la conjugate la 'mala oa burgundy le nang le recombinant rK39 antigen e kopantsoeng le khauta ea colloid (Leishmania conjugates) le li-conjugate tsa mmutla tsa IgG-gold;2) lesela la membrane la nitrocellulose le nang le lihlopha tse peli tsa liteko (lihlopha tsa M le G) le sehlopha sa taolo (C band).
Lisebelisoa / fanoeng | Bongata (Teko e 1/Kit) | Bongata(Litlhahlobo tse 5/Kit)
| Bongata(Liteko tse 25/Kit)
|
Teko Kit | 1 teko | 5 liteko | 25 liteko |
Buffer | 1 botlolo | 5 libotlolo | 25/2 libotlolo |
Drophara | 1 sengoathoana | 5 likhomphutha | 25 likhomphutha |
Mokotla oa Lipalangoang oa mohlala | 1 sengoathoana | 5 likhomphutha | 25 likhomphutha |
Lancet e lahliloeng | 1 sengoathoana | 5 likhomphutha | 25 likhomphutha |
Litaelo tsa ho Sebelisa | 1 sengoathoana | 1 sengoathoana | 1 sengoathoana |
Setifikeiti sa ho lumellana | 1 sengoathoana | 1 sengoathoana | 1 sengoathoana |
Bokella Serum ea motho/Plasma/Mali Aohle ka nepo.
Ka kopo bala litaelo ka hloko pele u etsa liteko.Pele u etsa tlhahlobo, lumella lisebelisoa tsa tlhahlobo, tharollo ea sampole le sampole hore li lekanngoe mochesong (15-30℃ kapa 59-86 degrees Fahrenheit).
1.Tlosa tube e ntšoang ka har'a kit le lebokose la teko ho tloha mokotleng oa filimi ka ho tabola notch.Li behe sefofaneng se rapameng.
2.Bula karete ea tlhahlobo ea aluminium foil bag.Tlosa karete ea teko 'me u e behe ka holimo tafoleng.
3.Sebelisa pipette e lahloang, fetisetsa lerotholi le le leng (hoo e ka bang 20μL) mali a monoana / kapa 4μL serum / kapa 4μL plasma / kapa 4μL mali a feletseng ka har'a sampuli selibeng sa cassette ea teko.
4.Bula buffer tube.Beha marotholi a 3 (hoo e ka bang 80 μL) a mokelikeli o hlapollang ka har'a mokelikeli o entsoeng ka sebopeho se chitja hantle.Qala ho bala.
Bala sephetho ka metsotso e 5-10.Liphetho ka mor'a metsotso e 10 ha li sebetse.
Sephetho se mpe
Haeba feela mohala oa taolo oa boleng o hlaha 'me mela ea ho lemoha G le M e sa bontše
Sephetho se Ntle
1.Ka bobeli mohala oa C oa taolo ea boleng le mohala oa ho lemoha M o hlaha= lesole la 'mele la Leishmania IgM lea fumanoa, 'me sephetho se nepahetse bakeng sa lesole la 'mele la IgM.
2.Bobeli mohala oa C oa taolo ea boleng le mohala oa ho lemoha G o hlaha= Leishmania IgG antibody e fumanoe 'me phello e ntle bakeng sa lesole la 'mele la IgG.
3.Ka bobeli mohala oa C oa taolo ea boleng le mela ea ho lemoha G le M e hlaha=lithibela-mafu tsa Leishmania IgG le IgM.li fumanoa, 'me phello e ntle bakeng sa li-antibodies tsa IgG le IgM.
Sephetho se fosahetseng
Mohala oa taolo ea boleng C o ke ke oa hlokomeloa, liphetho li tla be li sa sebetse ho sa tsotellehe hore na mohala oa teko o bontša, 'me tlhahlobo e lokela ho phetoa.
Lebitso la Sehlahiswa | Katse.Che | Boholo | Mohlala | Shelf Life | Trans.& Sto.Temp. |
Leishmania IgG/IgM Antibody Test Kit (Assay Immunochromatographic) | B020C-01 | 1 teko/kit | Serum/Plasma/Madi kaofela | 18 Likhoeli | 2-30 ℃ / 36-86 ℉ |
B020C-05 | 5 liteko/kit | ||||
B020C-25 | 25 liteko / kit |