Tšebeliso e reriloeng:
S. Typhi/Paratyphi Combo Antigen Rapid Test Kit (Immunochromatographic Assay) ke in vitro, quick, lateral flow test, e tsejoang hape e le lateral flow immunochromatographic assay, e reretsoeng ho lemoha boleng ba li-antigen tsa S. Typhi le Paratyphi ho mehlala ea mantle ho tloha bakuli.Liphetho tse tsoang ho S. Typhi/Paratyphi Combo Antigen Rapid Test Kit li lokela ho hlalosoa hammoho le tlhahlobo ea mokuli ea kliniki le mekhoa e meng ea ho hlahloba.
Melao-motheo ea Teko:
S. Typhi/Paratyphi Combo Antigen Rapid Test Kit(Immunochromatographic Assay) ke lateral flow chromatographic immunoassay.E na le mela e meraro ea pele ho coated, "T1" S. Typhi Test line, "T2" Paratyphi Test line le "C" Control line holim'a lera la nitrocellulose.Mouse monoclonal anti-S.Li-antibodies tsa typhi le anti-Paratyphi li koahetsoe sebakeng sa mohala oa teko 'me li-anti-chicken IgY tsa Pōli li koahetsoe sebakeng sa taolo. the S. Typhi/Paratyphi Antibody-labeled conjugate e etsa li-antigen-antibody particle complexes.Li-complexes li falla ka har'a lera la nitrocellulose ka ts'ebetso ea capillary ho fihlela mohala oa teko, moo li tšoaroang ke mouse monoclonal anti-S.Li-antibodies tsa typhi/Paratyphi.Mohala oa T1 o mebala o bonahala fensetereng ea sephetho haeba li-antigen tsa S. Typhi li le teng setšoantšong 'me matla a itšetlehile ka bongata ba S. Typhi antigen.Mohala oa T2 o nang le mebala o bonahala fensetereng ea sephetho haeba li-antigen tsa Paratyphi li le teng setšoantšong, 'me matla a itšetlehile ka palo ea antigen ea Paratyphi.Ha li-antigen tsa S.Typhi/Paratyphi tsa mohlala li le sieo kapa li le ka tlase ho moeli oa ho lemoha, ha ho na sehlopha sa mebala se bonahalang moleng oa Tlhahlobo (T1 le T2) ea sesebelisoa.Sena se bontša phello e mpe.Ha ho mohala oa teko kapa mohala oa taolo o hlahang fensetereng ea sephetho pele o sebelisa mohlala.Ho hlokahala mohala o bonahalang oa taolo ho bontša hore sephetho se nepahetse
Karolo REF REF | B033C-01 | B033C-05 | B033C-25 |
Leka Cassette | 1 teko | 5 liteko | 25 liteko |
Buffer | 1 botlolo | 5 botlolo | 25/2 libotlolo |
Mokotla oa Lipalangoang oa mohlala | 1 sengoathoana | 5 likhomphutha | 25 likhomphutha |
Litaelo tsa ho Sebelisa | 1 sengoathoana | 5 likhomphutha | 25 likhomphutha |
Setifikeiti sa ho lumellana | 1 sengoathoana | 1 sengoathoana | 1 sengoathoana |
Mohato oa 1: Mohlalae Tokisetso
1. Bokella mehlala ea mantle ka har'a linkho tse hloekileng, tse sa duleng.
2. Mohlala oa Lipalangoang le oa Polokelo: Mehlala e ka bolokoa mochesong oa kamore ka lihora tse 8 kapa ea beoa sehatsetsing ho 36°F ho ea ho 46°F (2°C ho isa ho 8°C) ho fihlela lihora tse 96.
3. Mehlala ea mantle e bolokiloeng e hoamisitsoe e ka qhibiliha ho fihlela makhetlo a 2 ho -10°C kapa ka tlaase ho moo.Haeba u sebelisa li-specimens tse leqhoa, qhibiliha mocheso oa kamore.Se ke oa lumella li-mantle ho lula ka har'a motsoako o hlapolotsoeng ka lihora tse fetang tse peli.
Mohato oa 2: Teko
1. Ka kopo bala litaelo ka hloko pele u etsa liteko.Pele u etsa liteko, lumella lik'hasete tsa teko, tharollo ea sampole le lisampole ho leka-lekana mocheso oa kamore (15-30 ℃ kapa 59-86 degrees Fahrenheit).
2. Ntša k’hasete ea teko ka mokotleng oa foil ’me u e behe sebakeng se bataletseng.
3. Hlakola botlolo ea mohlala, sebelisa thupa e khomaretsoeng e khomaretsoeng holim'a kepisi ho fetisetsa sampole e nyane ea mantle (3- 5 mm bophara; hoo e ka bang 30-50 mg) ka har'a botlolo ea sampole e nang le buffer ea ho lokisa mohlala.
4. Kenya thupa sebakeng sa botlolo ebe u tiisa ka mokhoa o sireletsehileng.Kopanya sampole ea setulo le buffer hantle ka ho tsukutla botlolo ka makhetlo a 'maloa ebe u tlohela tube ka metsotso e 2.
5. Hlakola ntlha ea botlolo ea mohlala 'me u tšoare botlolo sebakeng se otlolohileng holim'a seliba sa sampuli sa Cassette, fana ka marotholi a 3 (100 -120μL) a sampuli ea setulo se hlapolotsoeng ho ea sampuli selibeng.
Mohato oa 3: Ho bala
Bala liphetho ka metsotso e 15-20.Nako ea tlhaloso ea sephetho ha e fete metsotso e 20
1. S. Typhi Phello e Ntle
Lihlopha tse mebala li hlaha moleng oa teko ka bobeli (T1) le mohala oa taolo (C).E bontša sephetho se setle bakeng sa li-antigen tsa S. Typhi setšoantšong.
2. Paratyphi Positive Sephetho
Lihlopha tse mebala li hlaha moleng oa teko ka bobeli (T2) le mohala oa taolo (C).E bontša phello e ntle bakeng sa li-antigen tsa Paratyphi ka mohlala.
3. S. Typhi le Paratyphi Positive Sephetho
Lihlopha tse mebala li hlaha moleng oa teko ka bobeli (T1), mohala oa teko (T2) le mohala oa taolo (C).E bontša sephetho se setle bakeng sa li-antigen tsa S. Typhi le Paratyphi setšoantšong.
4. Sephetho se Fosahetseng
Sehlopha sa mebala se hlaha moleng oa taolo (C) feela.E bontša hore bongata ba S. Typhi kapa Paratyphi antigens ha bo teng kapa bo ka tlase ho tekanyo ea ho fumanoa ha tlhahlobo.
5. Sephetho se Fosahetseng
Ha ho na sehlopha sa mebala se bonahalang se hlahang moleng oa taolo ka mor'a ho etsa tlhahlobo.E ka 'na eaba litsela ha lia ka tsa lateloa hantle kapa tlhahlobo e ka 'na eaba e senyehile.Ho khothalletsoa hore mohlala o hlahlojoe hape
Lebitso la Sehlahiswa | Katse.Che | Boholo | Mohlala | Shelf Life | Trans.& Sto.Temp. |
S. Typhi/Paratyphi Combo Antigen Rapid Test Kit(Immunochromatographic Assay) | B033C-01 | 1 teko/kit | Fecal | Likhoeli tse 24 | 2-30 ℃ |
B033C-05 | 5 liteko/kit | ||||
B033C-25 | 25 liteko / kit |