Sehlomathiso sa tlhahlobo ea typhoid IgG/IgM Antibody (Immunochromatographic Assay)
Lintlha tsa sehlahisoa
Tšebeliso e reriloeng
Typhoid IgG/IgM Antibody Test Kit (Immunochromatographic Assay) e sebelisa mokhoa oa khauta oa colloidal ho lemoha ka mokhoa o nepahetseng antibody ea typhoid bacillus (lipopolysaccharide antigen le membrane e ka ntle ea protheine antigen) ho serum ea motho / plasma, e loketseng tlhahlobo ea pele ea typhoid. .
Teko Molao-motheo
Thiphoid IgG/IgM Antibody Test Kit (Immunochromatographic Assay) ke lateral flow chromatography immunoassay.Cassette ea teko e na le: 1) burgundy colored conjugate pad e nang le recombinant S. typhoid H antigen le O antigen e kopantsoeng le khauta ea colloid (typhoid conjugates) le li-conjugate tsa mmutla tsa IgG ‐ khauta, 2) lesela la nitrocellulose le nang le lihlopha tse peli tsa teko (Ig G). le lihlopha tsa IgM) le sehlopha sa taolo (C sehlopha).Sehlopha sa IgM se kentsoe pele ka monoclonal anti-human IgM bakeng sa ho lemoha IgM anti-S.typhi, sehlopha sa IgG se tlositsoe ka li-reagents bakeng sa ho lemoha IgG anti-S.typhi, 'me sehlopha sa C se koahetsoe pele ka pōli ea anti rabbit IgG.
Litaba Tse ka Sehloohong
Likarolo tse fanoeng li thathamisitsoe tafoleng.
| Lisebelisoa / fanoeng | Bongata(Teko e 1/Kit)
| Bongata(Litlhahlobo tse 5/Kit)
| Bongata(Liteko tse 25/Kit)
|
| Teko Kit | 1 teko | 5 liteko | 25 liteko |
| Buffer | 1 botlolo | 5 libotlolo | 25/2 libotlolo |
| Drophara | 1 sengoathoana | 5 sengoathoana | 25 karolo |
| Mokotla oa Lipalangoang oa mohlala | 1 sengoathoana | 5 likhomphutha | 25 likhomphutha |
| Lancet e lahliloeng | 1 sengoathoana | 5 likhomphutha | 25 likhomphutha |
| Litaelo tsa ho Sebelisa | 1 sengoathoana | 1 sengoathoana | 1 sengoathoana |
| Setifikeiti sa ho lumellana | 1 sengoathoana | 1 sengoathoana | 1 sengoathoana |
Phallo ea Ts'ebetso
Mohato oa 1: Mohlala
Bokella Serum ea motho/Plasma/Mali Aohle ka nepo.
Mohato oa 2: Teko
1. Tlosa tube ea ho ntša ka har'a kit le lebokose la teko ho tloha mokotleng oa filimi ka ho tabola notch.Bula mokotlana oa foil oa aluminium oa karete ea tlhahlobo.Tlosa karete ea teko 'me u li behe ka holimo tafoleng.
2. Sebelisa pipette e lahloang, fetisetsa 4μL serum (kapa plasma), kapa 4μL mali a feletseng ka har'a sampuli selibeng sa k'hasete ea teko.
3.Bula buffer tube ka ho sotha ho tloha holimo.Beha marotholi a 3 (hoo e ka bang 80 μL) a mokelikeli o hlapollang ka har'a mokelikeli o entsoeng ka sebopeho se chitja hantle.Qala ho bala.
Mohato oa 3: Ho bala
Bala sephetho ka 15 min.Liphetho ka mor'a metsotso e 20 ha li sebetse.
Tlhaloso ea Sephetho
Sephetho se mpe
Ke mohala oa C oa taolo ea boleng feela o hlahang 'me mela ea ho lemoha G le M ha e bontše, ho bolela hore ha ho anti-anti-typhoid e fumanoang 'me phello e mpe.
Sephetho se Ntle
Haeba ka bobeli mohala oa C oa taolo ea boleng le mohala oa ho lemoha M o hlaha= lesole la 'mele la Typhoid IgM le fumanoa, 'me sephetho se nepahetse bakeng sa lesole la 'mele la IgM.
Haeba ka bobeli mohala oa C oa taolo ea boleng le mohala oa ho lemoha G o hlaha= lesole la 'mele la Typhoid IgG le fumanoa 'me sephetho se nepahetse bakeng sa lesole la 'mele la IgG.
Haeba ka bobeli mohala oa C oa taolo ea boleng le mela ea ho lemoha G le M e hlaha= li-antibodies tsa Typhoid IgG le IgM li fumanoa, 'me sephetho se nepahetse bakeng sa li-antibodies tsa IgG le IgM ka bobeli.
Sephetho se fosahetseng
Haeba mohala oa C oa taolo ea boleng o ke ke oa bonoa, liphetho li tla be li sa sebetse
ho sa tsotellehe hore na mola oa teko o bontša, 'me teko e lokela ho phetoa.
Lintlha tsa Odara
| Lebitso la Sehlahiswa | Katse.Che | Boholo | Mohlala | Shelf Life | Trans.& Sto.Temp. |
| Typhoid Sesebelisoa sa tlhahlobo sa IgG/IgM Antibody (Assay ea Immunochromatographic) | B023C-01 | 1 teko/kit | Serum/Plasma/Madi kaofela | 18 Likhoeli | 2-30 ℃ / 36-86 ℉ |
| B023C-05 | 5 liteko/kit | ||||
| B023C-25 | 25 liteko / kit |
