Recombinant Monkeypox Virus A29L Protein, C-Tag ea hae

Lintlha tsa Sehlahisoa

Mohloli	Monkeypox Virus(strain Zaire-96-I-16)
Expression Host	E.coli
Tag	C- Letlapa la hae
Kopo	E loketse ho sebelisoa ho immunoassays. Laeborari e 'ngoe le e 'ngoe e lokela ho etsa qeto ea hore na ke boemo bofe bo nepahetseng ba ho sebetsa bakeng sa tšebeliso ea eona.
Lintlha tse akaretsang	Recombinant monkeypox virus A29L protheine e hlahisoa ke E.coli expression system le target gene encoding Met1-Glu110 e hlahisoa ka His-tag ho C-terminus.

Bohloeki	>95% joalo ka ha ho khethoa ke SDS-PAGE.
'Misa oa limolek'hule	Protheine e kopaneng ea monkeypox virus A29L e nang le li-amino acid tse 125 mme e na le boima ba molek'hule bo baliloeng ba 14.2 kDa.
Sehlahisoa Buffer	20 mM Tris, 300 mM NaCl, pH 9.0.
Polokelo	E boloke tlas'a maemo a nyopa ho -20 ℃ ho -80 ℃ ha e amoheloa. Khothalletsa ho aliquot protheine ka bongata bo nyane bakeng sa polokelo e nepahetseng.